Abstract : Air samples were collected during were from July 2009 to June 2011 along railwaylines of western suburban region of Mumbai and examined for fungal spores Concurrently by using gravity slide and petriplate exposure technique,. of these 27 Fungal types recorded. The significant types includes Alternaria, Aspergillus,Basidiospores,Bispora,Candida,Chaetomium,Cladosporium, Curvularia,Didymosphaeria,Epicoccum,Fusarium,Helminthosporium, Hypoxylon, Mucor, Mamniolla, Penicillium, Pithomyces, Trichonis, etc